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The Immune Synapse

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Cover of 'The Immune Synapse'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 The Immune Synapse: Past, Present, and Future
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    Chapter 2 Analyzing Actin Dynamics at the Immunological Synapse
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    Chapter 3 Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse
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    Chapter 4 Analyzing the Dynamics of Signaling Microclusters
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    Chapter 5 Reconstitution of TCR Signaling Using Supported Lipid Bilayers
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    Chapter 6 Plasma Membrane Sheets for Studies of B Cell Antigen Internalization from Immune Synapses
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    Chapter 7 Studying the Dynamics of TCR Internalization at the Immune Synapse
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    Chapter 8 T Cell Receptor Activation of NF-κB in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse
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    Chapter 9 Imaging Vesicular Traffic at the Immune Synapse
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    Chapter 10 Analysis of TCR/CD3 Recycling at the Immune Synapse
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    Chapter 11 Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses
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    Chapter 12 Mathematical Modeling of Synaptic Patterns
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    Chapter 13 Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy
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    Chapter 14 Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse
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    Chapter 15 Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes
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    Chapter 16 Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET
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    Chapter 17 Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning
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    Chapter 18 Spatial Control of Biological Ligands on Surfaces Applied to T Cell Activation
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    Chapter 19 Probing Synaptic Biomechanics Using Micropillar Arrays
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    Chapter 20 Microchannels for the Study of T Cell Immunological Synapses and Kinapses
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    Chapter 21 Purification of LAT-Containing Membranes from Resting and Activated T Lymphocytes
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    Chapter 22 Quantitative Phosphoproteomic Analysis of T-Cell Receptor Signaling
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    Chapter 23 Imaging Asymmetric T Cell Division
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    Chapter 24 Ultrastructure of Immune Synapses
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    Chapter 25 Systems Imaging of the Immune Synapse
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    Chapter 26 Comprehensive Analysis of Immunological Synapse Phenotypes Using Supported Lipid Bilayers
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    Chapter 27 Studying Immunoreceptor Signaling in Human T Cells Using Electroporation of In Vitro Transcribed mRNA
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    Chapter 28 A Protein Expression Toolkit for Studying Signaling in T Cells
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    Chapter 29 Imaging the Effector CD8 Synapse
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    Chapter 30 The Mast Cell Antibody-Dependent Degranulatory Synapse
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    Chapter 31 Measurement of Lytic Granule Convergence After Formation of an NK Cell Immunological Synapse
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    Chapter 32 Studying the T Cell-Astrocyte Immune Synapse
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    Chapter 33 Aberrant Immunological Synapses Driven by Leukemic Antigen-Presenting Cells
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    Chapter 34 Studying the Immune Synapse in HIV-1 Infection
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    Chapter 35 In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes
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    Chapter 36 Studying Dendritic Cell-T Cell Interactions Under In Vivo Conditions
Attention for Chapter 35: In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes
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Chapter title
In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes
Chapter number 35
Book title
The Immune Synapse
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6881-7_35
Pubmed ID
Book ISBNs
978-1-4939-6879-4, 978-1-4939-6881-7
Authors

Hélène D. Moreau, Philippe Bousso

Editors

Cosima T. Baldari, Michael L. Dustin

Abstract

T cells can become activated in lymph nodes following a diverse set of interactions with antigen-presenting cells. These cellular contacts range from short and dynamic to stable and long-lasting interactions, termed kinapses and synapses, respectively. Here, we describe a methodology to generate naïve T cells expressing a fluorescent probe of interest through the generation of bone marrow chimeras and to image T cell dynamics using intravital two-photon microscopy. In these settings, the formation of kinapses and synapses can be triggered by the administration of low and high affinity peptides, respectively. Finally, 3D cell tracking can help classify distinct T cell behaviors. These approaches should offer new possibilities for dissecting the process of T cell activation in vivo.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 33%
Student > Postgraduate 1 17%
Student > Ph. D. Student 1 17%
Unknown 2 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Agricultural and Biological Sciences 1 17%
Immunology and Microbiology 1 17%
Unknown 2 33%