Title |
Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat
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Published in |
BMC Plant Biology, December 2012
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DOI | 10.1186/1471-2229-12-243 |
Pubmed ID | |
Authors |
Xiaofei Zhang, Hui Jin, Yan Zhang, Dongcheng Liu, Genying Li, Xianchun Xia, Zhonghu He, Aimin Zhang |
Abstract |
Low-molecular-weight glutenin subunits (LMW-GS) strongly influence the bread-making quality of bread wheat. These proteins are encoded by a multi-gene family located at the Glu-A3, Glu-B3 and Glu-D3 loci on the short arms of homoeologous group 1 chromosomes, and show high allelic variation. To characterize the genetic and protein compositions of LMW-GS alleles, we investigated 16 Aroona near-isogenic lines (NILs) using SDS-PAGE, 2D-PAGE and the LMW-GS gene marker system. Moreover, the composition of glutenin macro-polymers, dough properties and pan bread quality parameters were determined for functional analysis of LMW-GS alleles in the NILs. |
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