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HisB as novel selection marker for gene targeting approaches in Aspergillus niger

Overview of attention for article published in BMC Microbiology, March 2017
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  • Above-average Attention Score compared to outputs of the same age (61st percentile)
  • Average Attention Score compared to outputs of the same age and source

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4 tweeters

Citations

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9 Dimensions

Readers on

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39 Mendeley
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Title
HisB as novel selection marker for gene targeting approaches in Aspergillus niger
Published in
BMC Microbiology, March 2017
DOI 10.1186/s12866-017-0960-3
Pubmed ID
Authors

Markus R. M. Fiedler, Tarek Gensheimer, Christin Kubisch, Vera Meyer

Abstract

For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene integration and deletion in A. niger. A histidine-auxotrophic strain was established via disruption of the A. niger hisB gene by using the counterselectable pyrG marker. After curing, a hisB (-) , pyrG (-) strain was obtained, which served as recipient strain for further studies. We show here that both hisB orthologs from A. nidulans and A. niger can be used to reestablish histidine prototrophy in this recipient strain. Whereas the hisB gene from A. nidulans was suitable for efficient gene targeting at different loci in A. niger, the hisB gene from A. niger allowed efficient integration of a Tet-on driven luciferase reporter construct at the endogenous non-functional hisB locus. Subsequent analysis of the luciferase activity revealed that the hisB locus is tight under non-inducing conditions and allows even higher luciferase expression levels compared to the pyrG integration locus. Taken together, we provide here an alternative selection marker for A. niger, hisB, which allows efficient homologous integration rates as well as high expression levels which compare favorably to the well-established pyrG selection marker.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 33%
Student > Master 6 15%
Researcher 5 13%
Student > Bachelor 4 10%
Other 2 5%
Other 5 13%
Unknown 4 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 38%
Agricultural and Biological Sciences 14 36%
Chemistry 2 5%
Immunology and Microbiology 1 3%
Business, Management and Accounting 1 3%
Other 0 0%
Unknown 6 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2017.
All research outputs
#3,698,005
of 9,189,847 outputs
Outputs from BMC Microbiology
#429
of 1,455 outputs
Outputs of similar age
#95,751
of 253,674 outputs
Outputs of similar age from BMC Microbiology
#19
of 35 outputs
Altmetric has tracked 9,189,847 research outputs across all sources so far. This one has received more attention than most of these and is in the 59th percentile.
So far Altmetric has tracked 1,455 research outputs from this source. They receive a mean Attention Score of 3.2. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 253,674 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.