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Auxins and Cytokinins in Plant Biology

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Cover of 'Auxins and Cytokinins in Plant Biology'

Table of Contents

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    Book Overview
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    Chapter 1 Methodological Advances in Auxin and Cytokinin Biology
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    Chapter 2 Analytical Determination of Auxins and Cytokinins
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    Chapter 3 Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae–Arabidopsis thaliana Interaction
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    Chapter 4 Microbial Manipulation of Auxins and Cytokinins in Plants
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    Chapter 5 A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling
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    Chapter 6 Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity
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    Chapter 7 Interplay Between Auxin and Cytokinin and Its Impact on Mitogen Activated Protein Kinase (MAPK)
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    Chapter 8 Quantification of Cytokinin Levels and Responses in Abiotic Stresses
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    Chapter 9 Assessment of Cytokinin-Induced Immunity Through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana
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    Chapter 10 Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies
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    Chapter 11 Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface
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    Chapter 12 Analyzing Cytokinin Responses During Plant-Nematode Interactions
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    Chapter 13 Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin
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    Chapter 14 A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks
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    Chapter 15 Monitoring of Crosstalk Between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots
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    Chapter 16 High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation
Attention for Chapter 15: Monitoring of Crosstalk Between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots
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Chapter title
Monitoring of Crosstalk Between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots
Chapter number 15
Book title
Auxins and Cytokinins in Plant Biology
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6831-2_15
Pubmed ID
Book ISBNs
978-1-4939-6829-9, 978-1-4939-6831-2
Authors

Víctor Carrasco Loba, Marta-Marina Pérez Alonso, Stephan Pollmann

Editors

Thomas Dandekar, Muhammad Naseem

Abstract

Over the last few years, it became more and more evident that plant hormone action is to great parts determined through their sophisticated crosstalk, rather than by their isolated activities. Thus, the parallel analysis of interconnected phytohormones in only very small amounts of tissue developed to an important issue in the field of plant sciences. In the following, a highly sensitive and accurate method is described for the quantitative analysis of the plant hormones jasmonic acid and indole-3-acetic acid in the model plant Arabidopsis thaliana. The described methodology is, however, not limited to the analysis of Arabidopsis samples but can also be applied to other plant species. The presented method is optimized for the working up of as little as 20-50 mg of plant tissue. Thus, it is well suited for the analysis of plant hormone contents in plant tissue of only little biomass, such as roots. The presented protocol facilitates the implementation of the method into other laboratories that have access to appropriate laboratory equipment and comparable state-of-the-art gas chromatography-mass spectrometry (GC-MS) technology.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Researcher 3 23%
Student > Bachelor 2 15%
Professor 1 8%
Professor > Associate Professor 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 38%
Biochemistry, Genetics and Molecular Biology 3 23%
Environmental Science 1 8%
Chemical Engineering 1 8%
Neuroscience 1 8%
Other 0 0%
Unknown 2 15%