| Chapter title |
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation
|
|---|---|
| Chapter number | 16 |
| Book title |
Auxins and Cytokinins in Plant Biology
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6831-2_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6829-9, 978-1-4939-6831-2
|
| Authors |
Nora Wehner, Jörn Herfert, Wolfgang Dröge-Laser, Christoph Weiste |
| Editors |
Thomas Dandekar, Muhammad Naseem |
| Abstract |
Genome sequencing and annotation studies clearly highlight the impact of transcriptional regulation in plants. However, functional characterization of the majority of transcriptional regulators remains elusive. Hence, high-throughput techniques are required to facilitate their molecular analysis. Here, we provide a detailed protocol to conduct a high-throughput protoplast trans-activation (PTA) screening, which enables simultaneous analysis of up to 95 individual transcription factor activities on a customizable promoter:LUCIFERASE reporter. This system is well suited to decipher complex transcriptional networks such as that triggered by the phytohormone auxin. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 10% |
| Professor | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 5 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 40% |
| Unspecified | 1 | 10% |
| Unknown | 5 | 50% |