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Prokaryotic Cytoskeletons

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Attention for Chapter 5: FtsZ Constriction Force – Curved Protofilaments Bending Membranes
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Chapter title
FtsZ Constriction Force – Curved Protofilaments Bending Membranes
Chapter number 5
Book title
Prokaryotic Cytoskeletons
Published in
Sub cellular biochemistry, May 2017
DOI 10.1007/978-3-319-53047-5_5
Pubmed ID
Book ISBNs
978-3-31-953045-1, 978-3-31-953047-5
Authors

Harold P. Erickson, Masaki Osawa

Editors

Jan Löwe, Linda A. Amos

Abstract

FtsZ assembles in vitro into protofilaments (pfs) that are one subunit thick and ~50 subunits long. In vivo these pfs assemble further into the Z ring, which, along with accessory division proteins, constricts to divide the cell. We have reconstituted Z rings in liposomes in vitro, using pure FtsZ that was modified with a membrane targeting sequence to directly bind the membrane. This FtsZ-mts assembled Z rings and constricted the liposomes without any accessory proteins. We proposed that the force for constriction was generated by a conformational change from straight to curved pfs. Evidence supporting this mechanism came from switching the membrane tether to the opposite side of the pf. These switched-tether pfs assembled "inside-out" Z rings, and squeezed the liposomes from the outside, as expected for the bending model. We propose three steps for the full process of cytokinesis: (a) pf bending generates a constriction force on the inner membrane, but the rigid peptidoglycan wall initially prevents any invagination; (b) downstream proteins associate to the Z ring and remodel the peptidoglycan, permitting it to follow the constricting FtsZ to a diameter of ~250 nm; the final steps of closure of the septum and membrane fusion are achieved by excess membrane synthesis and membrane fluctuations.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 37%
Student > Doctoral Student 3 10%
Student > Bachelor 3 10%
Student > Master 3 10%
Unspecified 1 3%
Other 2 7%
Unknown 7 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 33%
Agricultural and Biological Sciences 5 17%
Immunology and Microbiology 3 10%
Linguistics 1 3%
Unspecified 1 3%
Other 2 7%
Unknown 8 27%