Title |
BSTA: a targeted approach combines bulked segregant analysis with next- generation sequencing and de novo transcriptome assembly for SNP discovery in sunflower
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Published in |
BMC Genomics, September 2013
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DOI | 10.1186/1471-2164-14-628 |
Pubmed ID | |
Authors |
Maren Livaja, Yu Wang, Silke Wieckhorst, Grit Haseneyer, Michael Seidel, Volker Hahn, Steven J Knapp, Stefan Taudien, Chris-Carolin Schön, Eva Bauer |
Abstract |
Sunflower belongs to the largest plant family on earth, the genomically poorly explored Compositae. Downy mildew Plasmopara halstedii (Farlow) Berlese & de Toni is one of the major diseases of cultivated sunflower (Helianthus annuus L.). In the search for new sources of downy mildew resistance, the locus Pl(ARG)on linkage group 1 (LG1) originating from H. argophyllus is promising since it confers resistance against all known races of the pathogen. However, the mapping resolution in the Pl(ARG) region is hampered by significantly suppressed recombination and by limited availability of polymorphic markers. Here we examined a strategy developed for the enrichment of molecular markers linked to this specific genomic region. We combined bulked segregant analysis (BSA) with next-generation sequencing (NGS) and de novo assembly of the sunflower transcriptome for single nucleotide polymorphism (SNP) discovery in a sequence resource combining reads originating from two sunflower species, H. annuus and H. argophyllus. |
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Demographic breakdown
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Environmental Science | 1 | 1% |
Other | 0 | 0% |
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