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Synthetic Protein Switches

Overview of attention for book
Cover of 'Synthetic Protein Switches'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Synthetic Protein Switches: Theoretical and Experimental Considerations
  3. Altmetric Badge
    Chapter 2 Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange
  4. Altmetric Badge
    Chapter 3 Construction of Protein Switches by Domain Insertion and Directed Evolution
  5. Altmetric Badge
    Chapter 4 Catalytic Amyloid Fibrils That Bind Copper to Activate Oxygen
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    Chapter 5 Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors
  7. Altmetric Badge
    Chapter 6 Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modeling and Assessment
  8. Altmetric Badge
    Chapter 7 Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs
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    Chapter 8 Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases
  10. Altmetric Badge
    Chapter 9 Quantitative and Dynamic Imaging of ATM Kinase Activity
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    Chapter 10 Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains
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    Chapter 11 Protein and Protease Sensing by Allosteric Derepression
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    Chapter 12 DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation
  14. Altmetric Badge
    Chapter 13 Engineering and Characterizing Synthetic Protease Sensors and Switches
  15. Altmetric Badge
    Chapter 14 Characterizing Dynamic Protein–Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV
  16. Altmetric Badge
    Chapter 15 Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light
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    Chapter 16 Light-Regulated Protein Kinases Based on the CRY2-CIB1 System
  18. Altmetric Badge
    Chapter 17 Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
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    Chapter 18 Primer-Aided Truncation for the Creation of Hybrid Proteins
  20. Altmetric Badge
    Chapter 19 Engineering Small Molecule Responsive Split Protein Kinases
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    Chapter 20 Directed Evolution Methods to Rewire Signaling Networks
Attention for Chapter 3: Construction of Protein Switches by Domain Insertion and Directed Evolution
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

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Citations

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Chapter title
Construction of Protein Switches by Domain Insertion and Directed Evolution
Chapter number 3
Book title
Synthetic Protein Switches
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6940-1_3
Pubmed ID
Book ISBNs
978-1-4939-6938-8, 978-1-4939-6940-1
Authors

Lucas F. Ribeiro, Tiana D. Warren, Marc Ostermeier, Ribeiro, Lucas F., Warren, Tiana D., Ostermeier, Marc

Editors

Viktor Stein

Abstract

A protein switch is a protein that changes between inactive ("off") and active ("on") states in response to a biomolecule or physical signal. These switches can be created by fusing two domains in such a way that the activity of the output domain is regulated by the input domain's recognition of an input signal (such as the binding of a molecule, recognition of light). Here, we describe several methods for randomly fusing two domains to create domain insertion libraries from which protein switches can be identified by selections and/or screens.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 39%
Student > Ph. D. Student 4 17%
Unspecified 2 9%
Other 2 9%
Professor 1 4%
Other 1 4%
Unknown 4 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 35%
Agricultural and Biological Sciences 4 17%
Unspecified 2 9%
Nursing and Health Professions 2 9%
Neuroscience 2 9%
Other 0 0%
Unknown 5 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2020.
All research outputs
#7,523,397
of 22,959,818 outputs
Outputs from Methods in molecular biology
#2,335
of 13,136 outputs
Outputs of similar age
#121,264
of 308,059 outputs
Outputs of similar age from Methods in molecular biology
#40
of 304 outputs
Altmetric has tracked 22,959,818 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,136 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,059 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 304 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.