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Mendeley readers
Chapter title |
Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs
|
---|---|
Chapter number | 7 |
Book title |
Synthetic Protein Switches
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6940-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6938-8, 978-1-4939-6940-1
|
Authors |
Helen Farrants, Julien Hiblot, Rudolf Griss, Kai Johnsson |
Editors |
Viktor Stein |
Abstract |
Biosensors are used in many fields to measure the concentration of analytes, both in a cellular context and in human samples for medical care. Here, we outline the design of two types of modular biosensors: SNAP-tag-based indicators with a Fluorescent Intramolecular Tether (SNIFITs) and LUCiferase-based Indicators of Drugs (LUCIDs). These semisynthetic biosensors quantitatively measure analyte concentrations in vitro and on cell surfaces by an intramolecular competitive mechanism. We provide an overview of how to design and apply SNIFITs and LUCIDs. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 23% |
Researcher | 4 | 15% |
Student > Master | 4 | 15% |
Professor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 2 | 8% |
Unknown | 6 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 31% |
Chemistry | 6 | 23% |
Agricultural and Biological Sciences | 3 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Unknown | 8 | 31% |