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Synthetic Protein Switches

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Cover of 'Synthetic Protein Switches'

Table of Contents

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    Book Overview
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    Chapter 1 Synthetic Protein Switches: Theoretical and Experimental Considerations
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    Chapter 2 Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange
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    Chapter 3 Construction of Protein Switches by Domain Insertion and Directed Evolution
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    Chapter 4 Catalytic Amyloid Fibrils That Bind Copper to Activate Oxygen
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    Chapter 5 Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors
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    Chapter 6 Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modeling and Assessment
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    Chapter 7 Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs
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    Chapter 8 Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases
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    Chapter 9 Quantitative and Dynamic Imaging of ATM Kinase Activity
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    Chapter 10 Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains
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    Chapter 11 Protein and Protease Sensing by Allosteric Derepression
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    Chapter 12 DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation
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    Chapter 13 Engineering and Characterizing Synthetic Protease Sensors and Switches
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    Chapter 14 Characterizing Dynamic Protein–Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV
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    Chapter 15 Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light
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    Chapter 16 Light-Regulated Protein Kinases Based on the CRY2-CIB1 System
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    Chapter 17 Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
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    Chapter 18 Primer-Aided Truncation for the Creation of Hybrid Proteins
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    Chapter 19 Engineering Small Molecule Responsive Split Protein Kinases
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    Chapter 20 Directed Evolution Methods to Rewire Signaling Networks
Attention for Chapter 11: Protein and Protease Sensing by Allosteric Derepression
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Chapter title
Protein and Protease Sensing by Allosteric Derepression
Chapter number 11
Book title
Synthetic Protein Switches
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6940-1_11
Pubmed ID
Book ISBNs
978-1-4939-6938-8, 978-1-4939-6940-1
Authors

Hui Chin Goh, Farid J. Ghadessy, Saurabh Nirantar

Editors

Viktor Stein

Abstract

Peptide motifs are crucial mediators of protein-protein interactions as well as sites of specific protease activity. The detection and characterization of these events is therefore indispensable for a detailed understanding of cellular regulation. Here, we present versatile and modular sensors that allow the user to detect protease activity and protein-peptide interactions, as well as to screen for inhibitors using chromogenic, fluorescent, or luminescent output.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 33%
Unknown 2 67%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 2 67%