Title |
Evidence for the interaction of the human metapneumovirus G and F proteins during virus-like particle formation
|
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Published in |
Virology Journal, September 2013
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DOI | 10.1186/1743-422x-10-294 |
Pubmed ID | |
Authors |
Liat Hui Loo, Muhammad Raihan Jumat, Yi Fu, Teck Choon Ayi, Pui San Wong, Nancy WS Tee, Boon Huan Tan, Richard J Sugrue |
Abstract |
Human metapneumovirus (HMPV) is now a major cause of lower respiratory infection in children. Although primary isolation of HMPV has been achieved in several different cell lines, the low level of virus replication and the subsequent recovery of low levels of infectious HMPV have hampered biochemical studies on the virus. These experimental methodologies usually require higher levels of biological material that can be achieved following HMPV infection. In this study we demonstrate that expression of the HMPV F, G and M proteins in mammalian cells leads to HMPV virus-like particles (VLP) formation. This experimental strategy will serve as a model system to allow the process of HMPV virus assembly to be examined. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 34% |
Student > Master | 6 | 21% |
Researcher | 5 | 17% |
Student > Bachelor | 2 | 7% |
Professor | 1 | 3% |
Other | 2 | 7% |
Unknown | 3 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 45% |
Biochemistry, Genetics and Molecular Biology | 3 | 10% |
Immunology and Microbiology | 3 | 10% |
Medicine and Dentistry | 2 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 14% |
Unknown | 3 | 10% |