Chapter title |
miR-CATCH: MicroRNA Capture Affinity Technology.
|
---|---|
Chapter number | 23 |
Book title |
RNA Interference
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1538-5_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1537-8, 978-1-4939-1538-5
|
Authors |
Sebastian Vencken, Tidi Hassan, Noel G McElvaney, Stephen G J Smith, Catherine M Greene, Noel G. McElvaney, Stephen G. J. Smith, Catherine M. Greene, Vencken, Sebastian, Hassan, Tidi, McElvaney, Noel G., Smith, Stephen G. J., Greene, Catherine M. |
Abstract |
Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction. |
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Student > Ph. D. Student | 9 | 25% |
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Medicine and Dentistry | 1 | 3% |
Other | 0 | 0% |
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