↓ Skip to main content

The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes.

Overview of attention for article published in Journal of Molecular & Genetic Medicine, January 2012
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
33 Dimensions

Readers on

mendeley
42 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes.
Published in
Journal of Molecular & Genetic Medicine, January 2012
Pubmed ID
Authors

Francesca Ferrara, Eleonora Molesti, Eva Böttcher-Friebertshäuser, Giovanni Cattoli, Davide Corti, Simon D Scott, Nigel J Temperton, Ferrara F, Molesti E, Böttcher-Friebertshäuser E, Cattoli G, Corti D, Scott SD, Temperton NJ

Abstract

The monomer of influenza haemagglutinin is synthesized as a single polypeptide precursor that during maturation is cleaved by proteases into two active subunits. Other studies have demonstrated that the human Transmembrane Protease Serine 2 (TMPRSS2) can cleave the HA of human seasonal influenza viruses. Consequently, we have investigated the use of human Transmembrane Protease Serine 2 to produce high titre influenza haemmagglutinin (HA) lentiviral pseudotypes from Group 2 influenza viruses. Such pseudotypes represent powerful and safe tools to study viral entry and immune responses. Influenza pseudotype particles are obtained by co-transfecting human embryonic kidney HEK293T/17 cells using plasmids coding for the influenza HA, HIV gag-pol and a lentiviral vector incorporating firefly luciferase. However, in order to produce Group 2 pseudotypes, it was necessary to co-transfect a plasmid expressing the TMPRSS2 endoprotease, to achieve the necessary HA cleavage for infective particle generation. These lentiviral pseudotypes were shown to transduce HEK293T/17 cells with high efficiency. This demonstrates that TMPRSS2 is necessary for the functional activation, in vitro, of both the HA of human seasonal influenza and other Group 2 HA influenza strains. Additionally, we show that the Group 2 influenza pseudotype particles can be used as surrogate antigens in neutralization assays and are efficiently neutralized by corresponding influenza virus reference sera. These data demonstrate that the viral pseudotype system is a powerful method for serological surveillance of a wide range of influenza viruses.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 7%
Unknown 39 93%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 29%
Student > Ph. D. Student 10 24%
Researcher 9 21%
Student > Bachelor 3 7%
Professor > Associate Professor 2 5%
Other 5 12%
Unknown 1 2%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 40%
Engineering 5 12%
Immunology and Microbiology 4 10%
Biochemistry, Genetics and Molecular Biology 4 10%
Medicine and Dentistry 4 10%
Other 7 17%
Unknown 1 2%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2020.
All research outputs
#10,601,912
of 16,562,219 outputs
Outputs from Journal of Molecular & Genetic Medicine
#19
of 44 outputs
Outputs of similar age
#97,237
of 172,309 outputs
Outputs of similar age from Journal of Molecular & Genetic Medicine
#1
of 1 outputs
Altmetric has tracked 16,562,219 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 44 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.9. This one scored the same or higher as 25 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 172,309 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them