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The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

Overview of attention for article published in BMC Microbiology, March 2017
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Title
The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine
Published in
BMC Microbiology, March 2017
DOI 10.1186/s12866-017-0967-9
Pubmed ID
Authors

Amina Basic, Madeleine Blomqvist, Gunnar Dahlén, Gunnel Svensäter

Abstract

Hydrogen sulfide (H2S) is a toxic foul-smelling gas produced by subgingival biofilms in patients with periodontal disease and is suggested to be part of the pathogenesis of the disease. We studied the H2S-producing protein expression of bacterial strains associated with periodontal disease. Further, we examined the effect of a cysteine-rich growth environment on the synthesis of intracellular enzymes in F. nucleatum polymorphum ATCC 10953. The proteins were subjected to one-dimensional (1DE) and two-dimensional (2DE) gel electrophoresis An in-gel activity assay was used to detect the H2S-producing enzymes; Sulfide from H2S, produced by the enzymes in the gel, reacted with bismuth forming bismuth sulfide, illustrated as brown bands (1D) or spots (2D) in the gel. The discovered proteins were identified with liquid chromatography - tandem mass spectrometry (LC-MS/MS). Cysteine synthase and proteins involved in the production of the coenzyme pyridoxal 5'phosphate (that catalyzes the production of H2S) were frequently found among the discovered enzymes. Interestingly, a higher expression of H2S-producing enzymes was detected from bacteria incubated without cysteine prior to the experiment. Numerous enzymes, identified as cysteine synthase, were involved in the production of H2S from cysteine and the expression varied among Fusobacterium spp. and strains. No enzymes were detected with the in-gel activity assay among the other periodontitis-associated bacteria tested. The expression of the H2S-producing enzymes was dependent on environmental conditions such as cysteine concentration and pH but less dependent on the presence of serum and hemin.

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The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 65 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 14%
Student > Bachelor 7 11%
Student > Doctoral Student 5 8%
Student > Master 5 8%
Researcher 3 5%
Other 8 12%
Unknown 28 43%
Readers by discipline Count As %
Medicine and Dentistry 10 15%
Agricultural and Biological Sciences 7 11%
Biochemistry, Genetics and Molecular Biology 6 9%
Immunology and Microbiology 4 6%
Chemistry 2 3%
Other 7 11%
Unknown 29 45%