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Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution

Overview of attention for article published in Biotechnology for Biofuels, March 2017
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2 tweeters

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Title
Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution
Published in
Biotechnology for Biofuels, March 2017
DOI 10.1186/s13068-017-0753-9
Pubmed ID
Authors

Dujia Cheng, Dengjin Li, Yizhong Yuan, Lin Zhou, Xuyang Li, Tong Wu, Liang Wang, Quanyu Zhao, Wei Wei, Yuhan Sun

Abstract

Microalgae are highly efficient cellular factories that capture CO2 and are also alternative feedstock for biofuel production. Carbohydrates, proteins, and lipids are major biochemical components in microalgae. Carbohydrates or starch in microalgae are possible substrates in yeast fermentation for biofuel production. The carbon partitioning in microalgae could be regulated through environmental stresses, such as high concentration of CO2, high light intensity, and nitrogen starvation conditions. It is essential to obtain carbohydrate-rich microalgae via an optimal bioprocess strategy. The carbohydrate accumulation in a CO2 tolerance strain, Chlorella sp. AE10, was investigated with a two-stage process. The CO2 concentration, light intensity, and initial nitrogen concentration were changed drastically in both stages. During the first stage, it was cultivated over 3 days under 1% CO2, a photon flux of 100 μmol m(-2) s(-1), and 1.5 g L(-1) NaNO3. It was cultivated under 10% CO2, 1000 μmol m(-2) s(-1), and 0.375 g L(-1) NaNO3 during the second stage. In addition, two operation modes were compared. At the beginning of the second stage of mode 2, cells were diluted to 0.1 g L(-1) and there was no cell dilution in mode 1. The total carbohydrate productivity of mode 2 was increased about 42% compared with that of mode 1. The highest total carbohydrate content and the highest starch content of mode 2 were 77.6% (DW) and 60.3% (DW) at day 5, respectively. The starch productivity was 0.311 g L(-1) day(-1) and the total carbohydrate productivity was 0.421 g L(-1) day(-1) in 6 days. In this study, a novel two-stage process was proposed for improving carbohydrate and starch accumulation in Chlorella sp. AE10. Despite cell dilution at the beginning of the second stage, environmental stress conditions of high concentration of CO2, high light intensity, and limited nitrogen concentration at the second stage were critical for carbohydrate and starch accumulation. Although the cells were diluted, the growths were not inhibited and the carbohydrate productivity was improved. These results were helpful to establish an integrated approach from CO2 capture to biofuel production by microalgae.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Argentina 1 2%
Unknown 56 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 18 32%
Student > Ph. D. Student 9 16%
Student > Bachelor 8 14%
Researcher 7 12%
Unspecified 6 11%
Other 9 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 44%
Unspecified 7 12%
Biochemistry, Genetics and Molecular Biology 7 12%
Environmental Science 5 9%
Engineering 4 7%
Other 9 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2017.
All research outputs
#5,206,276
of 9,723,500 outputs
Outputs from Biotechnology for Biofuels
#443
of 842 outputs
Outputs of similar age
#137,267
of 261,196 outputs
Outputs of similar age from Biotechnology for Biofuels
#24
of 40 outputs
Altmetric has tracked 9,723,500 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 842 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 261,196 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.