Title |
Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization
|
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Published in |
Molecular Cytogenetics, October 2013
|
DOI | 10.1186/1755-8166-6-42 |
Pubmed ID | |
Authors |
Matthew D Genet, Ian M Cartwright, Takamitsu A Kato |
Abstract |
Fluorescence in situ Hybridization (FISH) utilizes peptide nucleic acid (PNA) probes to identify specific DNA sequences. Traditional techniques have required the heat denaturing of the DNA in formamide followed by multiple hours at moderated temperatures to allow the probe to hybridize to its specific target. Over the past 30 years, advancements in both protocols and probes have made FISH a more reliable technique for both biological research and medical diagnostics, additionally the protocol has been shortened to several minutes. These PNA probes were designed to target and hybridize to both DNA and RNA, and PNA-protein interactions still remain unclear. |
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