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Food Allergens

Overview of attention for book
Cover of 'Food Allergens'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Overview of the Commonly Used Methods for Food Allergens
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    Chapter 2 Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques
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    Chapter 3 Recombinant Allergen Production in E. coli
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    Chapter 4 Recombinant Allergens Production in Yeast
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    Chapter 5 2D-Electrophoresis and Immunoblotting in Food Allergy
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    Chapter 6 Two-Dimensional Electrophoresis and Identification by Mass Spectrometry
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    Chapter 7 Enzyme-Linked Immunosorbent Assay (ELISA)
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    Chapter 8 Detection of Food Allergens by Taqman Real-Time PCR Methodology
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    Chapter 9 Detection of Food Allergens by Phage-Displayed Produced Antibodies
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    Chapter 10 Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis
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    Chapter 11 Basophil Degranulation Assay
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    Chapter 12 Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy
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    Chapter 13 Assessment of IgE Reactivity of β-Casein by Western Blotting After Digestion with Simulated Gastric Fluid
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    Chapter 14 IgE Epitope Mapping Using Peptide Microarray Immunoassay
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    Chapter 15 T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens
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    Chapter 16 Tetramer-Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens
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    Chapter 17 T-Cell Epitope Prediction
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    Chapter 18 An Overview of Bioinformatics Tools and Resources in Allergy
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    Chapter 19 The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid-Loaded Dendritic Cells
  21. Altmetric Badge
    Chapter 20 Recent Advances in the Detection of Allergens in Foods
Attention for Chapter 11: Basophil Degranulation Assay
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  • Above-average Attention Score compared to outputs of the same age and source (63rd percentile)

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Chapter title
Basophil Degranulation Assay
Chapter number 11
Book title
Food Allergens
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6925-8_11
Pubmed ID
Book ISBNs
978-1-4939-6923-4, 978-1-4939-6925-8
Authors

Madhan Masilamani, Mohanapriya Kamalakannan, Hugh A. Sampson

Editors

Jing Lin, Marcos Alcocer

Abstract

Basophil degranulation assay has gained importance over the last decade in both diagnosis of food allergy and evaluation of progression of immunotherapy. This assay involves the identification and quantification of the expression of CD63 molecule on basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to cell membrane during the process of degranulation in which the contents of basophil granules are released. This chapter describes the methodology for performing this assay.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 33%
Student > Master 2 22%
Professor > Associate Professor 1 11%
Professor 1 11%
Unknown 2 22%
Readers by discipline Count As %
Medicine and Dentistry 2 22%
Agricultural and Biological Sciences 1 11%
Biochemistry, Genetics and Molecular Biology 1 11%
Economics, Econometrics and Finance 1 11%
Immunology and Microbiology 1 11%
Other 0 0%
Unknown 3 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2017.
All research outputs
#14,928,316
of 22,961,203 outputs
Outputs from Methods in molecular biology
#4,717
of 13,136 outputs
Outputs of similar age
#200,036
of 334,647 outputs
Outputs of similar age from Methods in molecular biology
#100
of 309 outputs
Altmetric has tracked 22,961,203 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,136 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,647 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 309 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.