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Mendeley readers
Chapter title |
Recombinant Allergen Production in E. coli
|
---|---|
Chapter number | 3 |
Book title |
Food Allergens
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6925-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
Authors |
Changqi Liu, LeAnna N. Willison, Shridhar K. Sathe |
Editors |
Jing Lin, Marcos Alcocer |
Abstract |
Recombinant protein allergens have been used in allergy studies, allergy diagnosis, and epitope mapping. Messenger RNAs (mRNAs) are isolated from tissues of interest for complementary DNA (cDNA) library construction. Subsequently, the allergen gene is amplified by polymerase chain reaction (PCR) and sequenced. The amplified gene is then cloned into an expression vector, expressed in Escherichia coli cells, and purified from the cell lysate. This chapter describes the protocols for recombinant allergen production. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 18% |
Student > Bachelor | 3 | 18% |
Other | 2 | 12% |
Researcher | 1 | 6% |
Professor > Associate Professor | 1 | 6% |
Other | 0 | 0% |
Unknown | 7 | 41% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 24% |
Biochemistry, Genetics and Molecular Biology | 3 | 18% |
Chemical Engineering | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Unknown | 8 | 47% |