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Mendeley readers
| Chapter title |
Recombinant Allergens Production in Yeast
|
|---|---|
| Chapter number | 4 |
| Book title |
Food Allergens
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6925-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
| Authors |
Maria Neophytou, Marcos Alcocer |
| Editors |
Jing Lin, Marcos Alcocer |
| Abstract |
The methylotropic yeast Pichia pastoris has been extensively used in large-scale production of properly folded recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins such as post-translational modifications, protein processing, and a reasonably sophisticated quality control of protein folding when compared against Escherichia coli. In this chapter, we describe the modified lab procedure for cloning and expression in Pichia pastoris of common food allergens sequences from the raw fruit to the fully folded biotinylated protein product. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 40% |
| Researcher | 1 | 20% |
| Student > Postgraduate | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Agricultural and Biological Sciences | 2 | 40% |
| Unknown | 1 | 20% |