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Mendeley readers
| Chapter title |
Two-Dimensional Electrophoresis and Identification by Mass Spectrometry
|
|---|---|
| Chapter number | 6 |
| Book title |
Food Allergens
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6925-8_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
| Authors |
Fernando de la Cuesta, Gloria Alvarez-Llamas, Maria G. Barderas |
| Editors |
Jing Lin, Marcos Alcocer |
| Abstract |
Two-dimensional electrophoresis (2-DE) is a classical separation technique for complex protein mixtures based on a first-separation step by isoelectric point (pI) followed by SDS-PAGE. Due to its high resolving power (including protein isoforms) and the capability to display post-translational modifications, 2-DE has been the technique of choice in many studies focused in characterizing allergens. In this chapter, we describe a standard protocol which enables efficient protein separation and subsequent protein identification by tandem mass spectrometry (MALDI-MS/MS). |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 33% |
| Lecturer | 1 | 33% |
| Student > Master | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 33% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |