Chapter title |
Detection of Food Allergens by Phage-Displayed Produced Antibodies
|
---|---|
Chapter number | 9 |
Book title |
Food Allergens
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6925-8_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
Authors |
Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, Teresa García |
Editors |
Jing Lin, Marcos Alcocer |
Abstract |
Phage display is a powerful tool to produce recombinant antibodies against a given antigen without animal immunization. This technology employs libraries of recombinant bacteriophages that display billions of different functional antibody fragments on their surface. They are selected by panning in vitro against the target antigen in search for specific binders. In this chapter, we describe the selection of single chain variable fragment (scFv) antibodies to be used for detection of allergenic proteins from nuts in food products. The artificial libraries TomLinson I+J (MRC Laboratory of Molecular Biology and MRC Centre for Protein Engineering) were employed that resulted in successful phage-ELISA systems for detection of almond and walnut proteins in commercial food products. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 3 | 20% |
Student > Bachelor | 2 | 13% |
Researcher | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Student > Doctoral Student | 1 | 7% |
Other | 2 | 13% |
Unknown | 3 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 33% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Veterinary Science and Veterinary Medicine | 1 | 7% |
Computer Science | 1 | 7% |
Engineering | 1 | 7% |
Other | 0 | 0% |
Unknown | 5 | 33% |