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Food Allergens

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Cover of 'Food Allergens'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of the Commonly Used Methods for Food Allergens
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    Chapter 2 Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques
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    Chapter 3 Recombinant Allergen Production in E. coli
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    Chapter 4 Recombinant Allergens Production in Yeast
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    Chapter 5 2D-Electrophoresis and Immunoblotting in Food Allergy
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    Chapter 6 Two-Dimensional Electrophoresis and Identification by Mass Spectrometry
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    Chapter 7 Enzyme-Linked Immunosorbent Assay (ELISA)
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    Chapter 8 Detection of Food Allergens by Taqman Real-Time PCR Methodology
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    Chapter 9 Detection of Food Allergens by Phage-Displayed Produced Antibodies
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    Chapter 10 Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis
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    Chapter 11 Basophil Degranulation Assay
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    Chapter 12 Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy
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    Chapter 13 Assessment of IgE Reactivity of β-Casein by Western Blotting After Digestion with Simulated Gastric Fluid
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    Chapter 14 IgE Epitope Mapping Using Peptide Microarray Immunoassay
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    Chapter 15 T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens
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    Chapter 16 Tetramer-Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens
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    Chapter 17 T-Cell Epitope Prediction
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    Chapter 18 An Overview of Bioinformatics Tools and Resources in Allergy
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    Chapter 19 The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid-Loaded Dendritic Cells
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    Chapter 20 Recent Advances in the Detection of Allergens in Foods
Attention for Chapter 9: Detection of Food Allergens by Phage-Displayed Produced Antibodies
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Chapter title
Detection of Food Allergens by Phage-Displayed Produced Antibodies
Chapter number 9
Book title
Food Allergens
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6925-8_9
Pubmed ID
Book ISBNs
978-1-4939-6923-4, 978-1-4939-6925-8
Authors

Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, Teresa García

Editors

Jing Lin, Marcos Alcocer

Abstract

Phage display is a powerful tool to produce recombinant antibodies against a given antigen without animal immunization. This technology employs libraries of recombinant bacteriophages that display billions of different functional antibody fragments on their surface. They are selected by panning in vitro against the target antigen in search for specific binders. In this chapter, we describe the selection of single chain variable fragment (scFv) antibodies to be used for detection of allergenic proteins from nuts in food products. The artificial libraries TomLinson I+J (MRC Laboratory of Molecular Biology and MRC Centre for Protein Engineering) were employed that resulted in successful phage-ELISA systems for detection of almond and walnut proteins in commercial food products.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 20%
Student > Bachelor 2 13%
Researcher 2 13%
Student > Ph. D. Student 2 13%
Student > Doctoral Student 1 7%
Other 2 13%
Unknown 3 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 33%
Biochemistry, Genetics and Molecular Biology 2 13%
Veterinary Science and Veterinary Medicine 1 7%
Computer Science 1 7%
Engineering 1 7%
Other 0 0%
Unknown 5 33%