| Chapter title |
T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens
|
|---|---|
| Chapter number | 15 |
| Book title |
Food Allergens
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6925-8_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
| Authors |
Madhan Masilamani, Mariona Pascal, Hugh A. Sampson |
| Editors |
Jing Lin, Marcos Alcocer |
| Abstract |
Characterization of allergen-specific T cells is critical to understand their contribution to disease pathogenesis. The identification of immunodominant T-cell epitopes is crucial for development of T-cell-based vaccines. Peptide-specific T-cell proliferation studies are usually performed in a library of short synthetic peptides (15mer or 20mer) with 3 or 5 offset spanning the entire length of the allergen. T-cell peptide epitopes lack the primary and tertiary structure of the native protein to cross-link IgE, but retain the ability to stimulate T cells. The peptides sequences can also be obtained either by in silico approaches and in vitro binding assays. The efficacy of T-cell epitope-based peptide immunotherapy has been proven in certain allergies. The present methodology describes T-cell proliferation assays using whole blood sample from allergic subjects. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 21% |
| Researcher | 2 | 14% |
| Professor | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Professor > Associate Professor | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 5 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 29% |
| Agricultural and Biological Sciences | 2 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Medicine and Dentistry | 1 | 7% |
| Unknown | 6 | 43% |