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CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway

Overview of attention for article published in Brazilian Journal of Medical and Biological Research, January 2017
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Title
CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway
Published in
Brazilian Journal of Medical and Biological Research, January 2017
DOI 10.1590/1414-431x20175861
Pubmed ID
Authors

T.Y. Long, R. Jing, F. Kuang, L. Huang, Z.X. Qian, T.L. Yang

Abstract

Myocardial ischemia is a major cause of death and remains a disease with extremely deficient clinical therapies and a major problem worldwide. Cold inducible RNA-binding protein (CIRBP) is reported to be involved in multiple pathological processes, including myocardial ischemia. However, the molecular mechanisms of myocardial ischemia remain elusive. Here, we first overexpressed CIRBP by transfection of pc-CIRBP (pcDNA3.1 containing coding sequenced for CIRBP) and silenced CIRBP by transfection of small interfering RNA targeting CIRBP (siCIRBP). pcDNA3.1 and the negative control of siCIRBP (siNC) were transfected into H9C2 cells to act as controls. We then constructed a cell model of myocardial ischemia through culturing cells in serum-free medium with hypoxia in H9C2 cells. Subsequently, AlamarBlue assay, flow cytometry and western blot analysis were used, respectively, to assess cell viability, reactive oxygen species (ROS) level and apoptosis, and expression levels of IκBα, p65 and Bcl-3. We demonstrated that CIRBP overexpression promoted cell proliferation (P<0.001), inhibited cell apoptosis (P<0.05), reduced ROS level (P<0.001), down-regulated phosphorylated levels of IκBα and p65 (P<0.01 or P<0.001), and up-regulated expression of Bcl-3 (P<0.001) in H9C2 cells with myocardial ischemia. The influence of CIRBP knockdown yielded opposite results. Our study revealed that CIRBP could protect H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway.

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Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Researcher 2 15%
Student > Master 1 8%
Lecturer 1 8%
Student > Postgraduate 1 8%
Other 0 0%
Unknown 4 31%
Readers by discipline Count As %
Medicine and Dentistry 4 31%
Biochemistry, Genetics and Molecular Biology 3 23%
Agricultural and Biological Sciences 1 8%
Nursing and Health Professions 1 8%
Unknown 4 31%