Chapter title |
Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora
|
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Chapter number | 6 |
Book title |
Meiosis
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6340-9_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6338-6, 978-1-4939-6340-9
|
Authors |
Bowring, Frederick J., Yeadon, P. Jane, Catcheside, David E. A., Frederick J. Bowring, P. Jane Yeadon, David E. A. Catcheside |
Abstract |
We have built a series of Neurospora crassa strains containing alleles of green fluorescent protein (GFP) to provide a visual phenotype for investigating meiotic recombination. These strains provide a convenient means of screening the Neurospora knockout library for genes involved in genetic recombination. They permit rapid analysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for GFP. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured. |
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