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Meiosis

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Cover of 'Meiosis'

Table of Contents

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    Book Overview
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    Chapter 1 Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae
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    Chapter 2 Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe
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    Chapter 3 Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast
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    Chapter 4 Ribosome Profiling for the Analysis of Translation During Yeast Meiosis
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    Chapter 5 Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation
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    Chapter 6 Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora
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    Chapter 7 High-Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae
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    Chapter 8 Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast
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    Chapter 9 In Vivo Imaging of Budding Yeast Meiosis
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    Chapter 10 Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I
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    Chapter 11 Flow Cytometry for the Isolation and Characterization of Rodent Meiocytes
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    Chapter 12 Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH
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    Chapter 13 Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy
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    Chapter 14 Live Imaging of Meiosis I in Late-Stage Drosophila melanogaster Oocytes
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    Chapter 15 Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes
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    Chapter 16 Drosophila Male Meiosis
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    Chapter 17 Analysis of Chromatin Dynamics During Drosophila Spermatogenesis
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    Chapter 18 Quantitative Modeling and Automated Analysis of Meiotic Recombination
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    Chapter 19 A Computational Approach to Study Gene Expression Networks
Attention for Chapter 13: Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy
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Chapter title
Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy
Chapter number 13
Book title
Meiosis
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6340-9_13
Pubmed ID
Book ISBNs
978-1-4939-6338-6, 978-1-4939-6340-9
Authors

Simon I. R. Lane, Stephen Crouch, Keith T. Jones

Abstract

Accurate chromosome segregation is necessary so that genetic material is equally shared among daughter cells. However, maturing mammalian oocytes are particularly prone to chromosome segregation errors, making them a valuable tool for identifying the causes of mis-segregation. Factors such as aging, cohesion loss, DNA damage, and the roles of a plethora of kinetochore and cell cycle-related proteins are involved. To study chromosome segregation in oocytes in a live setting is an imaging challenge that requires advanced techniques. Here we describe a method for examining chromosomes in live oocytes in detail as they undergo maturation. Our method is based on tracking the "center of brightness" of fluorescently labeled chromosomes. Here we describe how to set up our software and run experiments on a Leica TCS SP8 confocal microscope, but the method would be transferable to other microscopes with computer-aided microscopy.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 43%
Researcher 2 29%
Other 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Unknown 3 43%