Title |
Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation
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Published in |
PeerJ, March 2017
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DOI | 10.7717/peerj.3133 |
Pubmed ID | |
Authors |
Dorett I. Odoni, Juan A. Tamayo-Ramos, Jasper Sloothaak, Ruben G.A. van Heck, Vitor A.P. Martins dos Santos, Leo H. de Graaff, Maria Suarez-Diez, Peter J. Schaap |
Abstract |
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 3% |
Unknown | 31 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 6 | 19% |
Student > Bachelor | 6 | 19% |
Researcher | 5 | 16% |
Student > Ph. D. Student | 5 | 16% |
Professor > Associate Professor | 3 | 9% |
Other | 5 | 16% |
Unknown | 2 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 31% |
Chemical Engineering | 7 | 22% |
Biochemistry, Genetics and Molecular Biology | 7 | 22% |
Engineering | 4 | 13% |
Computer Science | 1 | 3% |
Other | 1 | 3% |
Unknown | 2 | 6% |