Title |
A Critical Review on the Use of Support Values in Tree Viewers and Bioinformatics Toolkits
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Published in |
Molecular Biology and Evolution, March 2017
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DOI | 10.1093/molbev/msx055 |
Pubmed ID | |
Authors |
Lucas Czech, Jaime Huerta-Cepas, Alexandros Stamatakis |
Abstract |
Phylogenetic trees are routinely visualized to present and interpret the evolutionary relationships of species. Most empirical evolutionary data studies contain a visualization of the inferred tree with branch support values. Ambiguous semantics in tree file formats can lead to erroneous tree visualizations and therefore to incorrect interpretations of phylogenetic analyses. Here, we discuss problems that arise when displaying branch values on trees after rerooting. Branch values are typically stored as node labels in the widely-used Newick tree format. However, such values are attributes of branches. Storing them as node labels can therefore yield errors when rerooting trees. This depends on the mostly implicit semantics that tools deploy to interpret node labels. We reviewed ten tree viewers and ten bioinformatics toolkits that can display and reroot trees. We found that 14 out of 20 of these tools do not permit users to select the semantics of node labels. Thus, unaware users might obtain incorrect results when rooting trees. We illustrate such incorrect mappings for several test cases and real examples taken from the literature. This review has already led to improvements in eight tools. We suggest tools should provide options that explicitly force users to define the semantics of node labels. |
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Geographical breakdown
Country | Count | As % |
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United States | 16 | 25% |
United Kingdom | 6 | 10% |
Germany | 3 | 5% |
France | 2 | 3% |
Sweden | 2 | 3% |
Brazil | 2 | 3% |
Netherlands | 2 | 3% |
China | 1 | 2% |
Australia | 1 | 2% |
Other | 5 | 8% |
Unknown | 23 | 37% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 40 | 63% |
Members of the public | 22 | 35% |
Unknown | 1 | 2% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | <1% |
Switzerland | 1 | <1% |
Mexico | 1 | <1% |
Russia | 1 | <1% |
Spain | 1 | <1% |
United States | 1 | <1% |
Unknown | 201 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 56 | 27% |
Student > Bachelor | 34 | 16% |
Researcher | 26 | 13% |
Student > Master | 24 | 12% |
Student > Doctoral Student | 9 | 4% |
Other | 23 | 11% |
Unknown | 35 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 90 | 43% |
Biochemistry, Genetics and Molecular Biology | 42 | 20% |
Environmental Science | 8 | 4% |
Immunology and Microbiology | 6 | 3% |
Computer Science | 5 | 2% |
Other | 10 | 5% |
Unknown | 46 | 22% |