Report for: Transgenic plants

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Chapter title	Characterization of rice genes using a heterologous full-length cDNA expression system.
Chapter number	8
Book title	Transgenic Plants
Published in	Methods in molecular biology, January 2012
DOI	10.1007/978-1-61779-558-9_8
Pubmed ID	22351001
Book ISBNs	978-1-61779-557-2, 978-1-61779-558-9
Authors	Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui, Higuchi, Mieko, Kondou, Youichi, Mori, Masaki, Ichikawa, Takanari, Matsui, Minami
Abstract	As a result of the progress in sequencing technology, many plant genomes have now been determined. Functional genomics is required to clarify gene function in many of these species. To identify useful genes easily and quickly, we have developed a FOX (full-length cDNA overexpressor) hunting system in which full-length cDNAs are overexpressed in Arabidopsis plants. This system was applied to high-throughput analysis of rice genes through heterologous expression in Arabidopsis (rice FOX Arabidopsis lines). We demonstrated that it is possible to carry out high-throughput analysis of gene function by utilizing rice FOX Arabidopsis lines. In this protocol, we describe how to isolate candidate rice FOX Arabidopsis lines and to determine the rice fl-cDNA that is responsible for the observed phenotype.

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Geographical breakdown

Country	Count	As %
United States	1	100%

Demographic breakdown

Type	Count	As %
Members of the public	1	100%

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	9	100%

Demographic breakdown

Readers by professional status	Count	As %
Researcher	5	56%
Student > Ph. D. Student	1	11%
Other	1	11%
Student > Master	1	11%
Unknown	1	11%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	4	44%
Environmental Science	1	11%
Energy	1	11%
Social Sciences	1	11%
Unknown	2	22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2012.

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#18,304,874

of 22,663,150 outputs

Outputs from Methods in molecular biology

#7,811

of 13,021 outputs

Outputs of similar age

#195,921

of 244,048 outputs

Outputs of similar age from Methods in molecular biology

#325

of 473 outputs

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Transgenic plants

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Geographical breakdown

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Attention Score in Context