Title |
Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach
|
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Published in |
Journal of Biological Engineering, October 2013
|
DOI | 10.1186/1754-1611-7-24 |
Pubmed ID | |
Authors |
Asif Rahman, Elisabeth Linton, Alex D Hatch, Ronald C Sims, Charles D Miller |
Abstract |
Polyhydroxyalkanoates (PHAs) are a group of biodegradable plastics that are produced by a wide variety of microorganisms, mainly as a storage intermediate for energy and carbon. Polyhydroxybutyrate (PHB) is a short-chain-length PHA with interesting chemical and physical properties. Large scale production of PHB is not wide-spread mainly due to the downstream processing of bacterial cultures to extract the PHB. Secretion of PHB from Escherichia coli could reduce downstream processing costs. PHB are non-proteinaceous polymers, hence cannot be directly targeted for secretion. Phasin, PhaP1, is a low molecular weight protein that binds to PHB, reducing PHB granule size. In this study PHB is indirectly secreted with PhaP1 from E. coli via type I secretion using HlyA signal peptides. |
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Demographic breakdown
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Researcher | 24 | 11% |
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Other | 20 | 9% |
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Environmental Science | 8 | 4% |
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Unknown | 57 | 27% |