Title |
RT-SHIV, an infectious CCR5-tropic chimeric virus suitable for evaluating HIV reverse transcriptase inhibitors in macaque models
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Published in |
AIDS Research and Therapy, November 2009
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DOI | 10.1186/1742-6405-6-23 |
Pubmed ID | |
Authors |
Yonghou Jiang, Baoping Tian, Mohammed Saifuddin, Michael B Agy, Peter Emau, J Scott Cairns, Che-Chung Tsai |
Abstract |
Non-nucleoside reverse transcriptase inhibitors (NNRTIs) are an important category of drugs for both chemotherapy and prevention of human immunodeficiency virus type 1 (HIV-1) infection. However, current non-human primate (NHP) models utilizing simian immunodeficiency virus (SIV) or commonly used chimeric SHIV (SIV expressing HIV-1 envelope) are inadequate due to the insensitivity to NNRTIs. To develop a NHP model for evaluation of NNRTI compounds, we characterized a RT-SHIV virus that was assembled by replacing the SIV mac239 reverse transcriptase (RT) with that of HIV-1HXB2. Since RT-SHIV exhibited in vitro characteristics of high infectivity, CCR5-usage, and sensitivity to HIV-1 specific NNRTIs, this virus was thought to be suitable for mucosal transmission and then was used to carry out a vaginal transmission study in pigtail macaques (Macaca nemestrina). |
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Members of the public | 1 | 100% |
Mendeley readers
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South Africa | 1 | 9% |
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