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RNA Methylation

Overview of attention for book
Cover of 'RNA Methylation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 LC-MS Analysis of Methylated RNA
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    Chapter 2 Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry
  4. Altmetric Badge
    Chapter 3 Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
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    Chapter 4 Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)
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    Chapter 5 Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
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    Chapter 6 Detection and Quantification of N 6-Methyladenosine in Messenger RNA by TLC
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    Chapter 7 Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)
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    Chapter 8 Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing
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    Chapter 9 Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
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    Chapter 10 Analysis of High-Throughput RNA Bisulfite Sequencing Data
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    Chapter 11 Statistical Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite Sequencing
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    Chapter 12 High-Throughput Mapping of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
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    Chapter 13 RiboMeth-seq: Profiling of 2′-O-Me in RNA
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    Chapter 14 In Silico Identification of RNA Modifications from High-Throughput Sequencing Data Using HAMR
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    Chapter 15 High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)
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    Chapter 16 Transcriptome-Wide Mapping of N 1-Methyladenosine Methylome
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    Chapter 17 In Vitro Assays for RNA Methyltransferase Activity
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    Chapter 18 Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo
  20. Altmetric Badge
    Chapter 19 Methylated mRNA Nucleotides as Regulators for Ribosomal Translation
  21. Altmetric Badge
    Chapter 20 Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA
Attention for Chapter 5: Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
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  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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Chapter title
Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
Chapter number 5
Book title
RNA Methylation
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6807-7_5
Pubmed ID
28349454
Book ISBNs
978-1-4939-6805-3, 978-1-4939-6807-7
Authors

Grozhik, Anya V., Linder, Bastian, Olarerin-George, Anthony O., Jaffrey, Samie R., Anya V. Grozhik, Bastian Linder, Anthony O. Olarerin-George, Samie R. Jaffrey

Editors

Alexandra Lusser

Abstract

N (6) -methyladenosine (m6A) is the most abundant modified base in eukaryotic mRNA and has been linked to diverse effects on mRNA fate. Current m6A mapping approaches localize m6A residues to 100-200 nt-long regions of transcripts. The precise position of m6A in mRNAs cannot be identified on a transcriptome-wide level because there are no chemical methods to distinguish between m6A and adenosine. Here, we describe a method for using anti-m6A antibodies to induce specific mutational signatures at m6A residues after ultraviolet light-induced antibody-RNA crosslinking and reverse transcription. Then, we describe how to use these mutational signatures to map m6A residues at nucleotide resolution. Taken together, our protocol allows for high-throughput detection of individual m6A residues throughout the transcriptome.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 110 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 110 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 20%
Researcher 20 18%
Student > Master 12 11%
Student > Bachelor 9 8%
Student > Doctoral Student 6 5%
Other 14 13%
Unknown 27 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 45 41%
Agricultural and Biological Sciences 25 23%
Medicine and Dentistry 3 3%
Immunology and Microbiology 3 3%
Chemistry 3 3%
Other 7 6%
Unknown 24 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2018.
All research outputs
#14,928,316
of 22,962,258 outputs
Outputs from Methods in molecular biology
#4,716
of 13,136 outputs
Outputs of similar age
#183,966
of 308,511 outputs
Outputs of similar age from Methods in molecular biology
#94
of 303 outputs
Altmetric has tracked 22,962,258 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,136 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,511 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 303 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.

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