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Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

Overview of attention for article published in BMC Genomics, November 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (63rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

Mentioned by

wikipedia
1 Wikipedia page

Citations

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30 Dimensions

Readers on

mendeley
90 Mendeley
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Title
Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
Published in
BMC Genomics, November 2016
DOI 10.1186/s12864-016-3331-9
Pubmed ID
Authors

Tomomi Aida, Shota Nakade, Tetsushi Sakuma, Yayoi Izu, Ayu Oishi, Keiji Mochida, Harumi Ishikubo, Takako Usami, Hidenori Aizawa, Takashi Yamamoto, Kohichi Tanaka

Abstract

Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1 and PITCh-directed donor vectors, we achieved convenient one-step knock-in of gene cassettes and floxed allele both in human cells and mouse zygotes. Our results provide a technical platform for high-throughput knock-in.

Mendeley readers

The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 1%
Unknown 89 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 21%
Student > Ph. D. Student 18 20%
Student > Master 10 11%
Other 10 11%
Student > Bachelor 9 10%
Other 24 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 33 37%
Agricultural and Biological Sciences 28 31%
Unspecified 12 13%
Medicine and Dentistry 6 7%
Neuroscience 5 6%
Other 6 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 April 2017.
All research outputs
#2,599,001
of 9,665,907 outputs
Outputs from BMC Genomics
#2,128
of 6,595 outputs
Outputs of similar age
#91,883
of 262,849 outputs
Outputs of similar age from BMC Genomics
#41
of 94 outputs
Altmetric has tracked 9,665,907 research outputs across all sources so far. This one has received more attention than most of these and is in the 57th percentile.
So far Altmetric has tracked 6,595 research outputs from this source. They receive a mean Attention Score of 4.2. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,849 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 94 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.