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Mendeley readers
Attention Score in Context
| Title |
Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
|
|---|---|
| Published in |
BMC Genomics, November 2016
|
| DOI | 10.1186/s12864-016-3331-9 |
| Pubmed ID | |
| Authors |
Tomomi Aida, Shota Nakade, Tetsushi Sakuma, Yayoi Izu, Ayu Oishi, Keiji Mochida, Harumi Ishikubo, Takako Usami, Hidenori Aizawa, Takashi Yamamoto, Kohichi Tanaka |
| Abstract |
Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1 and PITCh-directed donor vectors, we achieved convenient one-step knock-in of gene cassettes and floxed allele both in human cells and mouse zygotes. Our results provide a technical platform for high-throughput knock-in. |
Mendeley readers
The data shown below were compiled from readership statistics for 157 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | <1% |
| Unknown | 156 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 33 | 21% |
| Researcher | 30 | 19% |
| Student > Master | 20 | 13% |
| Student > Bachelor | 15 | 10% |
| Other | 10 | 6% |
| Other | 24 | 15% |
| Unknown | 25 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 59 | 38% |
| Agricultural and Biological Sciences | 40 | 25% |
| Neuroscience | 8 | 5% |
| Medicine and Dentistry | 8 | 5% |
| Engineering | 3 | 2% |
| Other | 9 | 6% |
| Unknown | 30 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 May 2023.
All research outputs
#2,569,250
of 23,539,593 outputs
Outputs from BMC Genomics
#820
of 10,789 outputs
Outputs of similar age
#52,433
of 420,042 outputs
Outputs of similar age from BMC Genomics
#26
of 251 outputs
Altmetric has tracked 23,539,593 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,789 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,042 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 251 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.