| Chapter title |
Gene Expression Analysis of Shoot Apical Meristem Cell Types
|
|---|---|
| Chapter number | 16 |
| Book title |
Plant Organogenesis
|
| Published in |
Methods in molecular biology, November 2012
|
| DOI | 10.1007/978-1-62703-221-6_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-220-9, 978-1-62703-221-6
|
| Authors |
Ram Kishor Yadav, Stephen Snipes, Thomas Girke, G. Venugopala Reddy |
| Editors |
Ive De Smet |
| Abstract |
Shoot apical meristems (SAMs) of higher plants harbor a set of stem-cells and provide cells for the development of all the above-ground biomass of plants. Most of the important pattern formation events such as maintenance of stem-cell identity, specification and differentiation of leaf/flower primordia, and temporal control of the transition from vegetative to reproductive program are determined in SAMs. Genetic analysis has revealed molecular and hormonal pathways involved in stem-cell maintenance, organ differentiation, and flowering time. However, limited information is available as to how different pathways interact with each other to function as a network in specifying different cell types and their function. Deciphering gene networks that underlie cell fate transitions requires new approaches aimed at assaying genome-scale expression patterns of genes at a single cell-type resolution. Here we provide details of experimental methods involved in protoplasting of SAM cells, generating cell type-specific gene expression profiles, and analysis platforms for identifying and inferring gene networks. |
X Demographics
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|---|---|---|
| Australia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 71% |
| Professor > Associate Professor | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 71% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |