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The focal adhesion targeting domain of p130Cas confers a mechanosensing function

Overview of attention for article published in Journal of Cell Science, February 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (61st percentile)
  • Above-average Attention Score compared to outputs of the same age and source (63rd percentile)

Mentioned by

twitter
4 tweeters

Citations

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2 Dimensions

Readers on

mendeley
11 Mendeley
Title
The focal adhesion targeting domain of p130Cas confers a mechanosensing function
Published in
Journal of Cell Science, February 2017
DOI 10.1242/jcs.192930
Pubmed ID
Authors

Peta M. Bradbury, Kylie Turner, Camilla Mitchell, Kaitlyn R. Griffin, Shiloh Middlemiss, Loretta Lau, Rebecca Dagg, Elena Taran, Justin Cooper-White, Ben Fabry, Geraldine M. O'Neill

Abstract

The Cas family of focal adhesion proteins contain a highly conserved C-terminal focal adhesion targeting (FAT) domain. To determine the role of the FAT domain we compared wildtype exogenous NEDD9 with a hybrid construct in which the NEDD9 FAT domain is exchanged for the p130Cas FAT domain. Fluorescence recovery after photobleaching (FRAP) revealed significantly slowed exchange of the fusion protein at focal adhesions and significantly slower 2D migration. No differences were detected in cell stiffness measured with Atomic Force Microscopy (AFM) and cell adhesion forces measured with a magnetic tweezer device. Thus the slowed migration was not due to changes in cell stiffness or adhesion strength. Analysis of cell migration on surfaces of increasing rigidity revealed a striking reduction of cell motility in cells expressing the p130Cas FAT domain. The p130Cas FAT domain induced rigidity-dependent tyrosine phosphorylation of the NEDD9 substrate domain. This in turn reduced post-translational cleavage of NEDD9 which we show inhibits NEDD9-induced migration. Collectively, our data therefore suggest that the p130Cas FAT domain uniquely confers mechanosensing function.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 3 27%
Professor > Associate Professor 3 27%
Student > Ph. D. Student 2 18%
Student > Doctoral Student 1 9%
Student > Master 1 9%
Other 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Unspecified 3 27%
Agricultural and Biological Sciences 3 27%
Medicine and Dentistry 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 August 2017.
All research outputs
#5,870,989
of 11,566,328 outputs
Outputs from Journal of Cell Science
#2,553
of 4,521 outputs
Outputs of similar age
#100,420
of 267,486 outputs
Outputs of similar age from Journal of Cell Science
#34
of 95 outputs
Altmetric has tracked 11,566,328 research outputs across all sources so far. This one is in the 48th percentile – i.e., 48% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,521 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,486 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.
We're also able to compare this research output to 95 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.