Title |
A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors
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Published in |
Microbial Cell Factories, October 2013
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DOI | 10.1186/1475-2859-12-100 |
Pubmed ID | |
Authors |
Alison Brognaux, Shanshan Han, Søren J Sørensen, Frédéric Lebeau, Philippe Thonart, Frank Delvigne |
Abstract |
Microbial cell population heterogeneity is now recognized as a major source of issues in the development and optimization of bioprocesses. Even if single cell technologies are available for the study of microbial population heterogeneity, only a few of these methods are available in order to study the dynamics of segregation directly in bioreactors. In this context, specific interfaces have been developed in order to connect a flow cytometer directly to a bioreactor for automated analyses. In this work, we propose a simplified version of such an interface and demonstrate its usefulness for multiplexed experiments. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Denmark | 1 | 1% |
China | 1 | 1% |
Unknown | 89 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 27 | 30% |
Student > Master | 16 | 18% |
Researcher | 10 | 11% |
Student > Bachelor | 8 | 9% |
Student > Doctoral Student | 5 | 5% |
Other | 12 | 13% |
Unknown | 13 | 14% |
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Agricultural and Biological Sciences | 28 | 31% |
Engineering | 13 | 14% |
Biochemistry, Genetics and Molecular Biology | 12 | 13% |
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Immunology and Microbiology | 3 | 3% |
Other | 13 | 14% |
Unknown | 16 | 18% |