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PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells

Overview of attention for article published in Nanoscale Research Letters, January 2011
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Title
PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells
Published in
Nanoscale Research Letters, January 2011
DOI 10.1186/1556-276x-6-447
Pubmed ID
Authors

Gao Liang, Yan Zhu, Bo Sun, Fei Hu, Tian, Shu Li, Zhong Xiao

Abstract

The biggest challenge in the field of gene therapy is how to effectively deliver target genes to special cells. This study aimed to develop a new type of poly(D,L-lactide-co-glycolide) (PLGA)-based nanoparticles for gene delivery, which are capable of overcoming the disadvantages of polyethylenimine (PEI)- or cationic liposome-based gene carrier, such as the cytotoxicity induced by excess positive charge, as well as the aggregation on the cell surface. The PLGA-based nanoparticles presented in this study were synthesized by emulsion evaporation method and characterized by transmission electron microscopy, dynamic light scattering, and energy dispersive spectroscopy. The size of PLGA/PEI nanoparticles in phosphate-buffered saline (PBS) was about 60 nm at the optimal charge ratio. Without observable aggregation, the nanoparticles showed a better monodispersity. The PLGA-based nanoparticles were used as vector carrier for miRNA transfection in HepG2 cells. It exhibited a higher transfection efficiency and lower cytotoxicity in HepG2 cells compared to the PEI/DNA complex. The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes. Moreover, nanocomplex shows better serum compatibility than commercial liposome. PLGA nanocomplexes obviously accumulate in tumor cells after transfection, which indicate that the complexes contribute to cellular uptake of pDNA and pronouncedly enhance the treatment effect of miR-26a by inducing cell cycle arrest. Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
Unknown 92 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 26%
Student > Master 16 17%
Student > Bachelor 15 16%
Student > Doctoral Student 9 10%
Researcher 7 8%
Other 13 14%
Unknown 9 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 33%
Medicine and Dentistry 13 14%
Engineering 10 11%
Pharmacology, Toxicology and Pharmaceutical Science 7 8%
Biochemistry, Genetics and Molecular Biology 6 6%
Other 16 17%
Unknown 10 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2011.
All research outputs
#3,108,413
of 4,506,407 outputs
Outputs from Nanoscale Research Letters
#97
of 424 outputs
Outputs of similar age
#36,528
of 54,840 outputs
Outputs of similar age from Nanoscale Research Letters
#3
of 6 outputs
Altmetric has tracked 4,506,407 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 424 research outputs from this source. They receive a mean Attention Score of 0.9. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 54,840 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.