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Histology Protocols

Overview of attention for chapter in “Histology Protocols”
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Chapter title
Histology Protocols
Book title
Histology Protocols
Published in
Methods in molecular biology, January 2010
DOI 10.1007/978-1-60327-345-9
Pubmed ID
Book ISBNs
978-1-60327-344-2, 978-1-60327-345-9
Authors

Chung JY, Hewitt SM, Joon-Yong Chung, Stephen M. Hewitt

Abstract

Formalin-fixed and paraffin-embedded (FFPE) tissue is one of the most valuable resources available for molecular biological analysis on tissue after diagnostic histopathological examination. Gene expression profiles of FFPE can provide insights into molecular mechanisms of disease but are difficult due to the high level of cross-linking of biomolecules by formalin fixation. Despite advances in molecular technologies, the quality of RNA obtained from FFPE tissue remains variable. We have optimized a reliable RNA extraction method for FFPE tissue. This approach is based on deparaffinization at high temperature coupled with a 3-day lysis at 65 degrees C. The average total RNA yield is 4.5-5.5 ng per 1 mm(3) of archival FFPE tissue and 260/280 ratios are between 1.80 and 1.95. The extracted RNA has a modal fragment length between 100 and 200 nucleotides by bioanalyzer analysis. Although modal lengths of RNA fragments were shorter, reverse transcription and polymerase chain reaction was able to amplify amplicons in the range of 300 base pairs. This optimized method improves the utility of FFPE tissue for molecular profiling studies.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Algeria 1 2%
Germany 1 2%
Unknown 43 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 15%
Student > Bachelor 4 9%
Researcher 4 9%
Professor > Associate Professor 3 7%
Other 2 4%
Other 4 9%
Unknown 22 48%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 28%
Medicine and Dentistry 5 11%
Biochemistry, Genetics and Molecular Biology 2 4%
Neuroscience 1 2%
Unknown 25 54%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2011.
All research outputs
#2,016,540
of 3,628,824 outputs
Outputs from Methods in molecular biology
#722
of 2,357 outputs
Outputs of similar age
#22,969
of 51,672 outputs
Outputs of similar age from Methods in molecular biology
#6
of 9 outputs
Altmetric has tracked 3,628,824 research outputs across all sources so far. This one is in the 25th percentile – i.e., 25% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,357 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 51,672 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.