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Autophagy protects against neural cell death induced by piperidine alkaloids present in Prosopis juliflora (Mesquite)

Overview of attention for article published in Anais da Academia Brasileira de Ciências, March 2017
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  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

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2 tweeters

Citations

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4 Dimensions

Readers on

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16 Mendeley
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Title
Autophagy protects against neural cell death induced by piperidine alkaloids present in Prosopis juliflora (Mesquite)
Published in
Anais da Academia Brasileira de Ciências, March 2017
DOI 10.1590/0001-3765201720160477
Pubmed ID
Authors

VICTOR D.A. SILVA, CARLOS CUEVAS, PATRICIA MUÑOZ, MONICA VILLA, ULISES AHUMADA-CASTRO, SANDRO HUENCHUGUALA, CLEONICE C. DOS SANTOS, FILLIPE M. DE ARAUJO, RAFAEL S FERREIRA, VANESSA B. DA SILVA, JULIANA H.C. E SILVA, ÉRICA N. SOARES, EUDES S. VELOZO, JUAN SEGURA-AGUILAR, SILVIA L. COSTA

Abstract

Prosopis juliflora is a shrub that has been used to feed animals and humans. However, a synergistic action of piperidine alkaloids has been suggested to be responsible for neurotoxic damage observed in animals. We investigated the involvement of programmed cell death (PCD) and autophagy on the mechanism of cell death induced by a total extract (TAE) of alkaloids and fraction (F32) from P. juliflora leaves composed majoritary of juliprosopine in a model of neuron/glial cell co-culture. We saw that TAE (30 µg/mL) and F32 (7.5 µg/mL) induced reduction in ATP levels and changes in mitochondrial membrane potential at 12 h exposure. Moreover, TAE and F32 induced caspase-9 activation, nuclear condensation and neuronal death at 16 h exposure. After 4 h, they induced autophagy characterized by decreases of P62 protein level, increase of LC3II expression and increase in number of GFP-LC3 cells. Interestingly, we demonstrated that inhibition of autophagy by bafilomycin and vinblastine increased the cell death induced by TAE and autophagy induced by serum deprivation and rapamycin reduced cell death induced by F32 at 24 h. These results indicate that the mechanism neural cell death induced by these alkaloids involves PCD via caspase-9 activation and autophagy, which seems to be an important protective mechanism.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 19%
Student > Doctoral Student 3 19%
Other 2 13%
Professor 1 6%
Student > Master 1 6%
Other 3 19%
Unknown 3 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 19%
Biochemistry, Genetics and Molecular Biology 2 13%
Engineering 2 13%
Neuroscience 2 13%
Computer Science 1 6%
Other 3 19%
Unknown 3 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2017.
All research outputs
#9,562,327
of 12,446,028 outputs
Outputs from Anais da Academia Brasileira de Ciências
#149
of 318 outputs
Outputs of similar age
#172,051
of 259,717 outputs
Outputs of similar age from Anais da Academia Brasileira de Ciências
#4
of 29 outputs
Altmetric has tracked 12,446,028 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 318 research outputs from this source. They receive a mean Attention Score of 2.0. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 259,717 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.