Title |
Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus
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Published in |
Clinics, February 2017
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DOI | 10.6061/clinics/2017(02)08 |
Pubmed ID | |
Authors |
Jianping Ma, Jufang Wang, Yanfen Liu, Changyi Wang, Donghui Duan, Nanjia Lu, Kaiyue Wang, Lu Zhang, Kaibo Gu, Sihan Chen, Tao Zhang, Dingyun You, Liyuan Han |
Abstract |
The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus. Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method. A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls. Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings. |
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United States | 1 | 100% |
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Members of the public | 1 | 100% |
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Researcher | 4 | 9% |
Student > Bachelor | 4 | 9% |
Student > Master | 4 | 9% |
Lecturer | 3 | 6% |
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Unknown | 15 | 32% |
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Economics, Econometrics and Finance | 1 | 2% |
Other | 3 | 6% |
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