| Chapter title |
Studying Protein–Protein Interactions In Planta Using Advanced Fluorescence Microscopy
|
|---|---|
| Chapter number | 17 |
| Book title |
Plant Genomics
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-7003-2_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7001-8, 978-1-4939-7003-2
|
| Authors |
Marc Somssich, Rüdiger Simon |
| Editors |
Wolfgang Busch |
| Abstract |
The formation of protein complexes through direct protein-protein interaction is essential for virtually every biological process, and accordingly the ability to determine the interaction properties of specific proteins is important to understand these processes. Förster resonance energy transfer (FRET) measurements are state-of-the-art confocal fluorescence microscopy- and imaging-based techniques that allow the analysis of protein interactions in vivo and in planta, in specific compartments of single cells or tissues. Here we provide a step-by-step guide to perform FRET measurements by acceptor photobleaching (APB) and fluorescence lifetime imaging microscopy (FLIM) in the plant expression system Nicotiana benthamiana. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 24% |
| Student > Ph. D. Student | 10 | 24% |
| Student > Master | 4 | 10% |
| Student > Doctoral Student | 3 | 7% |
| Professor | 3 | 7% |
| Other | 2 | 5% |
| Unknown | 9 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 39% |
| Biochemistry, Genetics and Molecular Biology | 8 | 20% |
| Chemistry | 6 | 15% |
| Unknown | 11 | 27% |