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Three novel Pseudomonas phages isolated from composting provide insights into the evolution and diversity of tailed phages

Overview of attention for article published in BMC Genomics, May 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

news
1 news outlet
twitter
14 tweeters
googleplus
1 Google+ user

Citations

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10 Dimensions

Readers on

mendeley
49 Mendeley
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Title
Three novel Pseudomonas phages isolated from composting provide insights into the evolution and diversity of tailed phages
Published in
BMC Genomics, May 2017
DOI 10.1186/s12864-017-3729-z
Pubmed ID
Authors

Deyvid Amgarten, Layla Farage Martins, Karen Cristina Lombardi, Luciana Principal Antunes, Ana Paula Silva de Souza, Gianlucca Gonçalves Nicastro, Elliott Watanabe Kitajima, Ronaldo Bento Quaggio, Chris Upton, João Carlos Setubal, Aline Maria da Silva

Abstract

Among viruses, bacteriophages are a group of special interest due to their capacity of infecting bacteria that are important for biotechnology and human health. Composting is a microbial-driven process in which complex organic matter is converted into humus-like substances. In thermophilic composting, the degradation activity is carried out primarily by bacteria and little is known about the presence and role of bacteriophages in this process. Using Pseudomonas aeruginosa as host, we isolated three new phages from a composting operation at the Sao Paulo Zoo Park (Brazil). One of the isolated phages is similar to Pseudomonas phage Ab18 and belongs to the Siphoviridae YuA-like viral genus. The other two isolated phages are similar to each other and present genomes sharing low similarity with phage genomes in public databases; we therefore hypothesize that they belong to a new genus in the Podoviridae family. Detailed genomic descriptions and comparisons of the three phages are presented, as well as two new clusters of phage genomes in the Viral Orthologous Clusters database of large DNA viruses. We found sequences encoding homing endonucleases that disrupt a putative ribonucleotide reductase gene and an RNA polymerase subunit 2 gene in two of the phages. These findings provide insights about the evolution of two-subunits RNA polymerases and the possible role of homing endonucleases in this process. Infection tests on 30 different strains of bacteria reveal a narrow host range for the three phages, restricted to P. aeruginosa PA14 and three other P. aeruginosa clinical isolates. Biofilm dissolution assays suggest that these phages could be promising antimicrobial agents against P. aeruginosa PA14 infections. Analyses on composting metagenomic and metatranscriptomic data indicate association between abundance variations in both phage and host populations in the environment. The results about the newly discovered and described phages contribute to the understanding of tailed bacteriophage diversity, evolution, and role in the complex composting environment.

Twitter Demographics

The data shown below were collected from the profiles of 14 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
Unknown 48 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 20%
Student > Ph. D. Student 10 20%
Student > Bachelor 7 14%
Researcher 5 10%
Professor > Associate Professor 4 8%
Other 4 8%
Unknown 9 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 29%
Agricultural and Biological Sciences 12 24%
Immunology and Microbiology 5 10%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Arts and Humanities 1 2%
Other 2 4%
Unknown 13 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 November 2018.
All research outputs
#1,025,325
of 15,348,483 outputs
Outputs from BMC Genomics
#305
of 8,696 outputs
Outputs of similar age
#29,451
of 266,684 outputs
Outputs of similar age from BMC Genomics
#2
of 7 outputs
Altmetric has tracked 15,348,483 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,696 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,684 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.