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Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonellaspp. in food

Overview of attention for article published in BMC Microbiology, December 2013
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Title
Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonellaspp. in food
Published in
BMC Microbiology, December 2013
DOI 10.1186/1471-2180-13-273
Pubmed ID
Authors

Baoguang Li, Jin-Qiang Chen

Abstract

Although a variety of methodologies are available for detection of Salmonella, sensitive, specific, and efficient methods are urgently needed for differentiation of live Salmonella cells from dead cells in food and environmental samples. Propidium monoazide (PMA) can preferentially penetrate the compromised membranes of dead cells and inhibit their DNA amplification, however, such inhibition has been reported to be incomplete by some studies. In the present study, we report an efficient qPCR assay targeting a conserved region of the invA gene of Salmonella in conjunction with PMA treatment for detection of DNA from live Salmonella cells in food samples.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 80 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
Norway 1 1%
Belgium 1 1%
Brazil 1 1%
Unknown 76 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 31%
Researcher 9 11%
Student > Master 8 10%
Student > Postgraduate 7 9%
Student > Bachelor 5 6%
Other 14 18%
Unknown 12 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 32 40%
Biochemistry, Genetics and Molecular Biology 12 15%
Immunology and Microbiology 4 5%
Environmental Science 4 5%
Veterinary Science and Veterinary Medicine 3 4%
Other 11 14%
Unknown 14 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2013.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from BMC Microbiology
#2,468
of 3,489 outputs
Outputs of similar age
#245,694
of 320,964 outputs
Outputs of similar age from BMC Microbiology
#34
of 49 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,489 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,964 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.