| Chapter title |
Ferroptosis and Cell Death Analysis by Flow Cytometry
|
|---|---|
| Chapter number | 6 |
| Book title |
Cell Viability Assays
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6960-9_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6959-3, 978-1-4939-6960-9, 978-1-4939-6959-3, 978-1-4939-6960-9
|
| Authors |
Daishi Chen, Ilker Y. Eyupoglu, Nicolai Savaskan |
| Editors |
Daniel F. Gilbert, Oliver Friedrich |
| Abstract |
Cell death and its recently discovered regulated form ferroptosis are characterized by distinct morphological, electrophysiological, and pharmacological features. In particular ferroptosis can be induced by experimental compounds and clinical drugs (i.e., erastin, sulfasalazine, sorafenib, and artesunate) in various cell types and cancer cells. Pharmacologically, this cell death process can be inhibited by iron chelators and lipid peroxidation inhibitors. Relevance of this specific cell death form has been found in different pathological conditions such as cancer, neurotoxicity, neurodegeneration, and ischemia. Distinguishing cell viability and cell death is essential for experimental and clinical applications and a key component in flow cytometry experiments. Dead cells can compromise the integrity of the data by nonspecific binding of antibodies and dyes. Therefore it is essential that dead cells are robustly and reproducibly identified and characterized by means of cytometry application. Here we describe a procedure to detect and quantify cell death and its specific form ferroptosis based on standard flow cytometry techniques. |
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Mendeley readers
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| Researcher | 3 | 5% |
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