| Chapter title |
High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity
|
|---|---|
| Chapter number | 4 |
| Book title |
Cell Viability Assays
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6960-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6959-3, 978-1-4939-6960-9
|
| Authors |
Delyan P. Ivanov, Anna M. Grabowska, Martin C. Garnett, Ivanov, Delyan P., Grabowska, Anna M., Garnett, Martin C. |
| Editors |
Daniel F. Gilbert, Oliver Friedrich |
| Abstract |
Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability assessment which use readily available reagents and open-source software to analyze spheroid volume, metabolism, and enzymatic activity. We provide two ImageJ macros for automated spheroid size determination-for both single images and images in stacks. We also share an Excel template spreadsheet allowing users to rapidly process spheroid size data, analyze plate uniformity (such as edge effects and systematic seeding errors), detect outliers, and calculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 26% |
| Student > Bachelor | 3 | 10% |
| Researcher | 3 | 10% |
| Student > Master | 3 | 10% |
| Student > Postgraduate | 2 | 6% |
| Other | 4 | 13% |
| Unknown | 8 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 26% |
| Medicine and Dentistry | 7 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 13% |
| Business, Management and Accounting | 2 | 6% |
| Agricultural and Biological Sciences | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 7 | 23% |