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Th9 Cells

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Cover of 'Th9 Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Th9 Cells: New Member of T Helper Cell Family
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    Chapter 2 IL-9: Function, Sources, and Detection
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    Chapter 3 IL-9 Signaling Pathway: An Update
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    Chapter 4 A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
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    Chapter 5 T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
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    Chapter 6 Polarizing Cytokines for Human Th9 Cell Differentiation
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    Chapter 7 Determining the Frequencies of Th9 Cells from Whole Blood
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    Chapter 8 IL-9 Production by Nonconventional T helper Cells
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    Chapter 9 Prediction and Validation of Transcription Factors Binding Sites in the Il9 Locus
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    Chapter 10 Flow Cytometric Assessment of STAT Molecules in Th9 Cells
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    Chapter 11 Transcription Factors Downstream of IL-4 and TGF-β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
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    Chapter 12 Retroviral Transduction and Reporter Assay: Transcription Factor Cooperation in Th9 Cell Development
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    Chapter 13 Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid Luminescent DNA Precipitation Assay
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    Chapter 14 Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation
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    Chapter 15 Allergic Inflammation and Atopic Disease: Role of Th9 Cells
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    Chapter 16 Characterization of Th9 Cells in the Development of EAE and IBD
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    Chapter 17 B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer
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    Chapter 18 Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models
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    Chapter 19 Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings
Attention for Chapter 4: A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
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Chapter title
A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
Chapter number 4
Book title
Th9 Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6877-0_4
Pubmed ID
Book ISBNs
978-1-4939-6876-3, 978-1-4939-6877-0
Authors

Duy Pham

Editors

Ritobrata Goswami

Abstract

CD4(+) T helper cells with the ability to produce effector cytokines provide host protection by regulating immune responses against pathogens. In contrast, these cells are responsible for the development of various inflammatory disease if not regulated properly. Previous studies using naïve CD4(+) T cell activation in vitro have shown the requirement of various cytokine combinations in addition to TCR activation to differentiate naïve CD4(+) T cells into various effector T helper lineages. The recently discovered CD4(+) T helper subset is IL-9-producing Th9 cells. Since Th9 cell differentiation in vitro is essential in understanding the molecular mechanism in regulating Th9 cell development, it is critical to develop a basic protocol in polarizing naïve CD4(+) T cells to Th9 cells in vitro. Here we describe a simple method for Th9 cell culture conditions in vitro that can be used for other molecular analyses.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 33%
Researcher 1 17%
Student > Bachelor 1 17%
Student > Ph. D. Student 1 17%
Librarian 1 17%
Other 0 0%
Readers by discipline Count As %
Immunology and Microbiology 2 33%
Agricultural and Biological Sciences 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Pharmacology, Toxicology and Pharmaceutical Science 1 17%
Medicine and Dentistry 1 17%
Other 0 0%