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Th9 Cells

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Table of Contents

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    Book Overview
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    Chapter 1 Th9 Cells: New Member of T Helper Cell Family
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    Chapter 2 IL-9: Function, Sources, and Detection
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    Chapter 3 IL-9 Signaling Pathway: An Update
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    Chapter 4 A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
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    Chapter 5 T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
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    Chapter 6 Polarizing Cytokines for Human Th9 Cell Differentiation
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    Chapter 7 Determining the Frequencies of Th9 Cells from Whole Blood
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    Chapter 8 IL-9 Production by Nonconventional T helper Cells
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    Chapter 9 Prediction and Validation of Transcription Factors Binding Sites in the Il9 Locus
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    Chapter 10 Flow Cytometric Assessment of STAT Molecules in Th9 Cells
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    Chapter 11 Transcription Factors Downstream of IL-4 and TGF-β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
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    Chapter 12 Retroviral Transduction and Reporter Assay: Transcription Factor Cooperation in Th9 Cell Development
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    Chapter 13 Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid Luminescent DNA Precipitation Assay
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    Chapter 14 Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation
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    Chapter 15 Allergic Inflammation and Atopic Disease: Role of Th9 Cells
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    Chapter 16 Characterization of Th9 Cells in the Development of EAE and IBD
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    Chapter 17 B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer
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    Chapter 18 Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models
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    Chapter 19 Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings
Attention for Chapter 8: IL-9 Production by Nonconventional T helper Cells
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Chapter title
IL-9 Production by Nonconventional T helper Cells
Chapter number 8
Book title
Th9 Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6877-0_8
Pubmed ID
Book ISBNs
978-1-4939-6876-3, 978-1-4939-6877-0, 978-1-4939-6876-3, 978-1-4939-6877-0
Authors

Silvia C. P. Almeida, Luis Graca M.D., D.Phil, Luis Graca

Editors

Ritobrata Goswami

Abstract

IL-9 is a pro-inflammatory cytokine implicated in certain immune-mediated diseases where chronic or acute inflammation of the mucosa plays an important role. Although initially described as being produced by what was then thought to be Th2 cells, it was later described that specialized lymphocyte populations are involved in IL-9 production. In addition to the classical Th9 effector (subset of CD4+ T cells), IL-9 is also produced by nonconventional lymphocytes, namely invariant natural killer T (iNKT) cells and innate lymphoid cells (ILCs). The identification of IL-9-producing cells by flow cytometry and cytokine measurements are pivotal for assigning and defining functional cellular phenotypes. In this chapter we provide methods for the in vitro polarization of IL-9-producing nonconventional lymphocytes and the best conditions for the detection of IL-9 production by intracellular staining.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 25%
Librarian 1 25%
Professor > Associate Professor 1 25%
Unspecified 1 25%
Readers by discipline Count As %
Immunology and Microbiology 2 50%
Unspecified 1 25%
Social Sciences 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 May 2017.
All research outputs
#8,518,974
of 9,792,785 outputs
Outputs from Methods in molecular biology
#5,068
of 7,512 outputs
Outputs of similar age
#219,296
of 263,520 outputs
Outputs of similar age from Methods in molecular biology
#17
of 35 outputs
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