| Chapter title |
T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
|
|---|---|
| Chapter number | 5 |
| Book title |
Th9 Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6877-0_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6876-3, 978-1-4939-6877-0
|
| Authors |
Françoise Meylan, Julio Gomez-Rodriguez |
| Editors |
Ritobrata Goswami |
| Abstract |
In vitro polarization of naïve CD4(+) T cells toward distinct T helper lineages is crucial for establishing the factors and features that determine the differentiation, stability, and effector function for each T helper subsets. In this regard, the recently defined Th9 subset has been reported with two essential cytokines requirement for their generation. Generating Th9 cells in vitro from naïve CD4(+) T cells requires the combination of TGF-β and IL-4. However, the amount of IL-9 producing under these minimal conditions is often small. The intent of this chapter is to provide examples to increase the generation of IL-9 producing T cells in vitro by modulating TCR strength and co-stimulation through the TNF family member TL1A. We hope that these methods to efficiently differentiate naïve CD4(+) T cells toward IL-9 producing cells will facilitate understanding the differentiation and function of Th9 cells and their pathogenesis in various inflammatory and autoimmune diseases. |
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|---|---|---|
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