| Chapter title |
Flow Cytometric Assessment of STAT Molecules in Th9 Cells
|
|---|---|
| Chapter number | 10 |
| Book title |
Th9 Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6877-0_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6876-3, 978-1-4939-6877-0
|
| Authors |
Lucien P. Garo, Vanessa Beynon, Gopal Murugaiyan, Garo, Lucien P., Beynon, Vanessa, Murugaiyan, Gopal |
| Editors |
Ritobrata Goswami |
| Abstract |
IL-9-producing Th9 cells are a novel subset of T helper cells that develop independently of other T helper subsets. Th9 cells have been implicated in the pathogenesis of allergic asthma and autoimmunity, while also serving as critical effector T cells in mediating antitumor immune responses. Concomitant presence of TGF-β and IL-4 lead to the differentiation of naïve CD4(+) T cells towards the Th9 phenotype. In addition, several cytokines, including IL-1β, IL-2, IL-25, and IL-33, further amplify Th9 responses. Negative regulators of Th9 cells include other cytokines such as IFN-γ, IL-23, and IL-27. Here, we describe a detailed protocol for the analysis of STAT molecules involved in the differentiation of Th9 cells and Th9 inhibition by IL-27. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 33% |
| Student > Master | 1 | 33% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
| Immunology and Microbiology | 1 | 33% |
| Unknown | 1 | 33% |