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Th9 Cells

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Cover of 'Th9 Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Th9 Cells: New Member of T Helper Cell Family
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    Chapter 2 IL-9: Function, Sources, and Detection
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    Chapter 3 IL-9 Signaling Pathway: An Update
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    Chapter 4 A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
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    Chapter 5 T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
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    Chapter 6 Polarizing Cytokines for Human Th9 Cell Differentiation
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    Chapter 7 Determining the Frequencies of Th9 Cells from Whole Blood
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    Chapter 8 IL-9 Production by Nonconventional T helper Cells
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    Chapter 9 Prediction and Validation of Transcription Factors Binding Sites in the Il9 Locus
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    Chapter 10 Flow Cytometric Assessment of STAT Molecules in Th9 Cells
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    Chapter 11 Transcription Factors Downstream of IL-4 and TGF-β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
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    Chapter 12 Retroviral Transduction and Reporter Assay: Transcription Factor Cooperation in Th9 Cell Development
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    Chapter 13 Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid Luminescent DNA Precipitation Assay
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    Chapter 14 Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation
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    Chapter 15 Allergic Inflammation and Atopic Disease: Role of Th9 Cells
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    Chapter 16 Characterization of Th9 Cells in the Development of EAE and IBD
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    Chapter 17 B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer
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    Chapter 18 Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models
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    Chapter 19 Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings
Attention for Chapter 13: Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid Luminescent DNA Precipitation Assay
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Chapter title
Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid Luminescent DNA Precipitation Assay
Chapter number 13
Book title
Th9 Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6877-0_13
Pubmed ID
Book ISBNs
978-1-4939-6876-3, 978-1-4939-6877-0, 978-1-4939-6876-3, 978-1-4939-6877-0
Authors

Etienne Humblin, François Ghiringhelli, Frédérique Végran

Editors

Ritobrata Goswami

Abstract

CD4+ T cells are key components of the immune system that shape the anticancer immune response in animal models and in humans among other responses. The biology of CD4+ T cells is complex because naïve CD4+ T cells can differentiate into various subpopulations with various functions that depend on the milieu. Recently, a new population of IL-9-secreting T cells called Th9 cells has been described. These cells are characterized by their ability to produce IL-9. These cells were described to be involved in parasite infections and allergic inflammation. However, some reports described their presence in the tumor bed in mice and humans. Molecular events that account for Th9 effector properties and differentiation are still elusive. To study these mechanisms, downregulation of gene expression and protein-DNA interaction detection allow to identify and study putative transcription factors regulating gene expression during their differentiation. Here, we describe three methods allowing the study of transcription factors in Th9 cells: siRNA transfection for downregulation of gene expression, and chromatin immunoprecipitation and liquid luminescent DNA precipitation assay to detect protein-DNA interactions.

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Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 May 2017.
All research outputs
#8,519,003
of 9,792,785 outputs
Outputs from Methods in molecular biology
#5,068
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Outputs of similar age
#219,318
of 263,520 outputs
Outputs of similar age from Methods in molecular biology
#17
of 35 outputs
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