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Chronic Chagas disease: PCR-xenodiagnosis without previous microscopic observation is a useful tool to detect viable Trypanosoma cruzi

Overview of attention for article published in Biological Research, January 2013
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Title
Chronic Chagas disease: PCR-xenodiagnosis without previous microscopic observation is a useful tool to detect viable Trypanosoma cruzi
Published in
Biological Research, January 2013
DOI 10.4067/s0716-97602013000300011
Pubmed ID
Authors

Miguel Saavedra, Inés Zulantay, Werner Apt, Gabriela Martínez, Antonio Rojas, Jorge Rodríguez

Abstract

We evaluate the elimination of the microscopic stage of conventional xenodiagnosis (XD) to optimize the parasitological diagnosis of Trypanosoma cruzi in chronic Chagas disease. To this purpose we applied under informed consent two XD cages to 150 Chilean chronic chagasic patients. The fecal samples (FS) of the triatomines at 30, 60 and 90 days post feeding were divided into two parts: in one a microscopic search for mobile trypomastigote and/or epimastigote forms was performed. In the other part, DNA extraction-purification for PCR directed to the conserved region of kDNA minicircles of trypanosomes (PCR-XD), without previous microscopic observation was done. An XD was considered positive when at least one mobile T. cruzi parasite in any one of three periods of incubation was observed, whereas PCR-XD was considered positive when the 330 bp band specific for T. cruzi was detected. 25 of 26 cases with positive conventional XD were PCR-XD positive (concordance 96.2%), whereas 85 of 124 cases with negative conventional XD were positive by PCR-XD (68.5%). Human chromosome 12 detected by Real-time PCR used as exogenous internal control of PCR-XD reaction allowed to discounting of PCR inhibition and false negative in 40 cases with negative PCR-XD. Conclusion: PCR-XD performed without previous microscopic observation is a useful tool for detection of viable parasites with higher efficiency then conventional XD.

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 23%
Student > Ph. D. Student 5 16%
Researcher 4 13%
Student > Bachelor 4 13%
Professor > Associate Professor 2 6%
Other 4 13%
Unknown 5 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 23%
Medicine and Dentistry 5 16%
Pharmacology, Toxicology and Pharmaceutical Science 2 6%
Biochemistry, Genetics and Molecular Biology 2 6%
Immunology and Microbiology 2 6%
Other 5 16%
Unknown 8 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2013.
All research outputs
#4,219,739
of 5,072,588 outputs
Outputs from Biological Research
#70
of 80 outputs
Outputs of similar age
#106,951
of 132,122 outputs
Outputs of similar age from Biological Research
#4
of 6 outputs
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So far Altmetric has tracked 80 research outputs from this source. They receive a mean Attention Score of 1.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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